CD4 and neoplasm: Considering the observation that in tumor microenvironment of Dox-treated mice both CD4+Foxp3− and CD8+ populations displayed enhanced cytokine production, we performed in vitro suppression assays with both CD4+ and CD8+ T naive populations in combination with sorted Treg cells from PBS-treated or Dox-treated animals (Supplementary Fig. 10a, b).