Here we showed that the disruption of D2R-DAT interaction by TAT-DATNT peptide stimulates locomotor behavior in normal and dopamine-depleted Sprague-Dawley rats by increasing extracellular dopamine, suggesting the TAT-DATNT peptide might also exert beneficial effects in the animal model of ADHD. The gene discussed is SLC6A3; the disease is attention deficit-hyperactivity disorder.