To further investigate the underlying mechanisms linked with the novel CPVT RyR2-D3638A mutation, we measured the intracellular Ca2+ handling properties at the excitation-contraction coupling level in Fluo-4 loaded hiPSC-CMs using confocal laser scanning microscopy. This evidence concerns the gene RYR2 and catecholaminergic polymorphic ventricular tachycardia.