5-AZ treatment can upregulate the expression of SPI1 and its target HAVCR2 but downregulate c-MYC expression in CEM cells, similar to the effects of our blast treatment, while no change can be detected in KE-37 cells (Figure 10C), demonstrating that SPI1 expression is also regulated by DNA methylation in human T-ALL. This evidence concerns the gene MYC and acute lymphoblastic leukemia.