Higher median ECPKA‐Ab levels were detected in the dogs with diagnoses of MMGT (7270 ng/mL), lymphoma (6418 ng/mL), HCC (8275) ng/mL, TCC (6310 ng/mL), HSA (5820 ng/mL), PAC (6258 ng/mL), and Mel (6100 ng/mL).Dog breed did not appear to have a significant effect on the ECPKA‐Ab level in dogs with or without cancer (P = 0.621 and P = 0.204, respectively). This evidence concerns the gene ALB and hepatocellular carcinoma.