Among these, the PALB2:c.104T>C (p.Leu35Pro), located in the coiled-coil domain, was found to co-segregate with two breast cancer cases in a family with a strong history for the disease, and was shown to abrogate the BRCA1-PALB2 binding and to completely prevent HR and resistance to DNA damaging agents. This evidence concerns the gene PALB2 and breast cancer.