TXNIP and hepatocellular carcinoma: We next analyzed the cell cycle of hepatocellular carcinoma cell line HuH‐7 overexpressing pAcGFP–TXNIP and its mutants as illustrated in Fig. 4A. Since HuH‐7 cells do not express TXNIP at a level detectable by western blot 15, the effects of endogenous TXNIP were negligible in this experiment.