In particular, our laboratory has demonstrated that Ewing sarcoma cells expressing high levels of ALDH, as determined by cell sorting using Aldefluor (ALDHhigh cells), exhibit a variety of stem cell properties, including clonogenic growth in soft agar, the ability to grow as spheres under non-adherent conditions, and expression of so-called “stem cell genes” such as OCT4 and NANOG [3]. This evidence concerns the gene LDHA and Ewing sarcoma.