To assess the impact of USP17 on NSCLC proliferation, we transfected A549 (EGFRWT), HCC827 (DelE746-A750) and H1975 (L858R/T790M) cells with either of two validated USP17 specific shRNAs (shRNA1 and shRNA2) (Fig. 1A/B/C) [7–9] or a non-targeting shRNA. The gene discussed is USP17L2; the disease is non-small cell lung carcinoma.