Therefore, to investigate if USP17 is required for the endocytosis and trafficking of mutant EGFRs in NSCLC cells we transfected the HCC827 and H1975 cells with shRNAs targeting USP17 and examined the localisation of EGFR using an anti-EGFR antibody after 15 min 2 ng/ml EGF treatment (Fig. 2). The gene discussed is USP17L2; the disease is non-small cell lung carcinoma.