TRIM59 and breast cancer: To examine the biological function of TRIM59 in breast cancer, we employed a short hairpin RNA (shRNA)-based knockdown (KD by 75%, Fig 2B) or clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein-9 nuclease (CRISPR/Cas9)-based knockout (KO, guanine- thymine [GT] insertion, Fig 2B) strategy in TRIM59-high breast cancer cell line MCF7 (Figs 2A and S2A and S2B).