In the xenograft models, overexpression of TRIM59 in MCF7 or MDA-MB-231 cells promoted pulmonary metastasis of breast cancer cells, whereas TRIM59 deficiency suppressed cancer cell metastasis, as reflected by cytokeratin 8 (CK8) staining of metastasized cancer cells in the lung (Fig 4I). This evidence concerns the gene KRT8 and cancer.