To test whether PDCD10-ROCK signaling is downstream of TRIM59 but upstream of MLC/ERM to impact breast cancer cells’ contractility (Fig 4), we performed functional rescue experiments by using a ROCK inhibitor (Y-27632) or through the lentivirus-mediated stable expression of PDCD10 (Fig 6F) in TRIM59 KO MCF7 cells. Here, MLC1 is linked to breast carcinoma.