To further explore the effect of TMZ on F5/35‐ZD55‐IL‐24‐mediated delivery of IL‐24 into melanoma cells, A375 and MV3 cells were treated with ZD55‐IL‐24, F5/35‐ZD55‐IL‐24, TMZ, ZD55‐IL‐24 + TMZ, or F5/35‐ZD55‐IL‐24 + TMZ. The gene discussed is IL24; the disease is melanoma.