We found that there was a similar level of the adenoviral E1A protein between the F5/35‐ZD55‐IL‐24 group and the F5/35‐ZD55‐IL‐24 + TMZ group (Figure 3A), which indicated that F5/35‐ZD55‐IL‐24 replicated effectively in melanoma cells and that TMZ did not increase the infectivity of CRAds. Here, DHTKD1 is linked to melanoma.