Using immunohistochemistry, we found that F5/35‐ZD55‐IL‐24 in combination with TMZ inhibited the expression of Bcl‐2, increased Bax, and upregulated pro‐caspase‐3, which suggested that F5/35‐ZD55‐IL‐24 in conjunction with TMZ could increase the levels of pro‐apoptotic proteins and decrease the level of anti‐apoptotic proteins in melanoma cells. Here, IL24 is linked to melanoma.