Given the fact that, over 80% of the KRAS mutations in both primary and metastatic tumors were observed as missense mutation in codons 12 and 13 and these mutations involved 17 mutation types (Figure 1), we decided to develop a mutation screening technique based on snapback primer high resolution melting analysis, which can potentially identify all the gene alterations in the codons 12 and 13 of KRAS gene. This evidence concerns the gene KRAS and metastatic neoplasm.