IL-8 and Annexin-A2 levels were measured in amniotic fluid that developed PB <32 weeks either with or without preterm premature rupture of membranes (PPROM); and combination of amniotic fluid IL-8 and Annexin-A2 for predicting PB within 2 weeks was reported to have a sensitivity of 81.25%, specificity of 88.89%, and positive predicting value (PPV) of 92.86% [68]. The gene discussed is ANXA2; the disease is spontaneous preterm birth.