TRPV4 and brain ischemia: The present study was aimed at exploring the relationship between the protective effect of TFR on ischemic brain injury and the function of TRPV4, SKca, and IKca channels with exclusion of the role of NO and PGI2 under both in vivo and in vitro situations in rat models of global cerebral ischemia and reperfusion in order to further explore the new mechanism and strategies for prevention of cerebral ischemia injury.