TNF and ischemia: The numbers of TNF-α single-positive and TNF-α+/Ly6C+ double-positive cells were 31 ± 7.87 and 1.0 ± 1.73 per view field in the ischemia vehicle group, and the proportion of Ly6C+ cells coexpressing TNF-α was 0.9 ± 1.2% (Figures 5(k) and 5(l)).