BDNF and ischemia: We performed double immunohistochemistry for BDNF and Ly6C (Figure 3) and found that, two days after ischemia onset, BDNF expression was enhanced in infiltrating Ly6C+ cells in the ischemia core cortex as compared with the sham control group, and only in the infarct area was BDNF staining found overlapped with Ly6C (Figures 3(a)–3(d)).