However, the levels of hepatic TNF-α (Figure 3A), IL-1β (Figure 3B), IL-4 (Figure 3C), IL-6 (Figure 3D), and TGF-β1 (Figure 3F) were significantly lower in rats with 2-AAF/PH-induced liver injury treated with Pue and RAPA than in untreated rats with liver injury, and IL-10 expression was higher (Figure 3E). This evidence concerns the gene TGFB1 and injury.