Next, Kcnmb2 expression in the hippocampus was quantified by qPCR: AAV-Kcnmb2 increased Kcnmb2 expression in the hippocampus of both CD and MD F1 mice compared to AAV-control virus (Figure 3B; Two-way ANOVA with the between-subjects factors AAV treatment and paternal diet, AAV treatment F(1,16) = 38.43, P < 0.0001; paternal diet F(1,16) = 0.05, P > 0.05; paternal diet × AAV treatment F(1,16) = 1.74, P > 0.05; Tukey’s multiple comparisons test, P < 0.01 for CD-Kcnmb2 vs. CD-control and P < 0.01 for MD-Kcnmb2 vs. MD-control). Here, KCNMB2 is linked to Menkes disease.