Method: A combination of array analysis data, in-silico prediction tools and nuclear magnetic resonance (NMR) system were used to analyse gene (mRNA) and miR expression, as well as metabolic changes associated with different rounds of knock in/out mutations in Apc, Kras, and p53 CRC mouse models and their tumour-derived organoids (TDOs). The gene discussed is KRAS; the disease is colorectal carcinoma.