To confirm the RNA-seq results, the mRNA levels of Ncoa4, Igf1r, Fgfr3, and Zbtb16, which belong to the “Pathways in cancer”, were validated by RT-qPCR, and collectively, the expression of these genes is increased in the Sirt2−/− mice (Fig. 8a). The gene discussed is ZBTB16; the disease is cancer.