Loss or depletion of PRKAA1/Prkaa1 in ECs results in a low level of glycolysis as well as accelerated atherosclerosis whereas the rescue of impaired glycolysis in Prkaa1-deficient ECs through the overexpression of Slc2a1 restores endothelial proliferation and protects against accelerated atherosclerosis. Here, PRKAA1 is linked to atherosclerosis.