The results showed that all BEPn complexes induced overexpression of HMGB1 in ER+ MCF-7 cells compared with ER− HCC-1937 cells, and BEP3 was nearly 2-fold more cytotoxic to ER+ MCF-7 cells than it was to ER− HCC-1937 cells. This evidence concerns the gene HMGB1 and hepatocellular carcinoma.