Methylation of TMEM176A was significantly associated with tumor cell differentiation (P < 0.05, Table 1), while no association was found between TMEM176A methylation and age, gender, HBV infection, liver cirrhosis, tumor size, lymph node metastasis, vessel cancerous embolus, number of lesions, and TNM stage. The gene discussed is TMEM176A; the disease is cirrhosis of liver.