MMP9 and cholangiocarcinoma: However, we found 100 nM apatinib was enough for CCA cell lines QBC939 and TFK-1 to cause migation and invasion inhibition (p < 0.01; Fig. 3b, and p < 0.05, p < 0.01, respectively; Fig. 3c), Furthermore, metastatic marker Slug, snail and MMP9 protein levels in the cells treated with or without 100 nM apatinib were detected by western blot.