Although strong anti-tumor effects of the hGPC1-CART were shown in a xenograft model implanted with GPC-1+ human cancer cells, this model was not sufficient to evaluate adverse effects of CART due to severe xenoGVHD and to evaluate induction of CTL against endogenous tumor antigens, which may be important for augmenting anti-tumor effects and overcoming antigen-loss problem in solid tumors. The gene discussed is GPC1; the disease is neoplasm.