(4) Cytokine expression profiling of NGY-A treated tumors by RT-PCR indicated an increase in anti-tumor cytokines IFNγ, TNFα, IL-1β and decrease in pro-tumor cytokines TGFβ, IL-10 compared to control group tumors suggesting that NGY-A treatment can increase anti-tumor CD8+ T-cells, M1 macrophages and suppress tumor promoting M2-phenotype. This evidence concerns the gene TNF and neoplasm.