Samples that were heterogeneous (determined by bracket evaluation with H&E staining), had high melanin content, or failed to meet minimum criteria (≥100 tumor cells per 28-8 assay) were excluded from the final analysis.For the primary analysis, overall, positive, and negative percentage agreement (OPA, PPA, and NPA) at the ≥1% PD-L1 expression level were evaluated. This evidence concerns the gene CD274 and neoplasm.