PDCD1 and neoplasm: Multiplexed fluorescence immunohistochemistry combined with unique AQUA (Automated Quantitative Analysis) algorithms specifically designed to classify regulatory T cells (Tregs), Myeloid derived suppressor cells (MDSCs), CD3+ T cells, PD-1 expression, PD-L1 expression and IDO1/HLA-DR co-expression was used to assess tumor immune modulation in response to neoadjuvant immunotherapy.