C57BL/6 mice bearing murine GPC-1 transduced MC38 (MC38-mGPC1) or MCA205 (MCA205-mGPC1) were treated with mGPC1-CART, and antitumor effects, adverse effects, induction of endogenous tumor antigen specific T cells, and combination with anti-PD-1 Ab were evaluated. Here, PDCD1 is linked to neoplasm.