Intra-assay reproducibility and quantification was accomplished by manually staining 5 serial sections from three different tissue types (tonsil, melanoma, NSCLC) with one set for each of the UltiMapper PD-1 (CD3, CD45RO, PD-1, CK/Sox10) and PD-L1 (CD8, CD68, PD-L1, CK/Sox10) I/O kits. This evidence concerns the gene CD8A and non-small cell lung carcinoma.