113 formalin-fixed, paraffin-embedded (FFPE) tumor samples of metastatic NSCLC were evaluated by RNA-seq to measure transcript levels of genes related to cell proliferation, DNA-seq of 409 genes for tumor mutational burden (TMB), and PD-L1 status (Dako 22C3 antibody assay). The gene discussed is CD274; the disease is neoplasm.