20 non-small cell lung carcinomas (9 adenocarcinomas (ADC), 11 squamous cell carcinomas (SCC) were stained with a 6-marker mIF panel (PD-L1, CD8, Ki-67, CD68, pancytokeratins, and PD-1) and imaged using a multispectral scanner. Here, PDCD1 is linked to squamous cell carcinoma.