45 formalin fixed, paraffin embedded NSCLC resection samples were stained with a custom-developed 7-plex mIF panel (CD68, CD8, Ki67, PD1, PD-L1, pancytokeratins-CK & DAPI) using the Opal method (PerkinElmer). Here, MKI67 is linked to non-small cell lung carcinoma.