One tumor was resected 2 days before treatment (pre-TX) and the other was treated with RT (3X8 Gy) or anti-CTLA-4 antibody (3x200 μg i.p.)monotherapy or in combination and resected 1 day after treatment when immune-mediated tumor rejection is occurring in tumors treated with RT+anti- CTLA-4 (post-TX). The gene discussed is CTLA4; the disease is neoplasm.