CD274 and non-small cell lung carcinoma: 113 formalin-fixed, paraffin-embedded (FFPE) tumor samples of metastatic NSCLC were evaluated by RNA-sequencing to measure transcript levels of genes related to tumor infiltrating lymphocytes and cell proliferation, DNA-sequencing of 409 genes for tumor mutational burden (TMB), and PD-L1 status (Dako 22C3 antibody assay).