Two new OpalTM reagents (Opal 480 and Opal 780) were combined with currently available Opal 7-color kits to stain and distinguish 8 markers plus DAPI when imaged on the Vectra Polaris®.Figure 1 shows a 9-color panel on lung cancer: CD20 (Opal 480), PD-L1 (Opal 520), CD8 (Opal 540), FoxP3 (Opal 570), CD68 (Opal 620), PD-1 (Opal 650), Ki67 (Opal 690), and PanCK (Opal 780). The gene discussed is CD274; the disease is lung cancer.