qPCR validation of the microarray analysis in murine adrenocortical tumors treated with CTX vs CT showed downregulation of Gata4 (Fig. 7A), Lhcgr (Fig. 7B), Cyclin A1 (Ccna1) (Fig. 7C) and upregulation of extracellular matrix compounds such as Sgcd (Fig. 7D), Mmp24 (Fig. 7E); genes related to cell growth suppression, Grb10 (Fig. 7F), Rerg (Fig. 7G); G0/G1 Switch 2 (G0s2) (Fig. 7H); Tumor Suppressor Candidate 5 (Tusc5) (Fig. 7I) or GPCR-mediated Ras protein-specific guanine nucleotide-releasing factor 1 (Rasgrf2) (Fig. 7J). This evidence concerns the gene RASGRF1 and adrenal cortex neoplasm.