qPCR validation of the microarray analysis in murine adrenocortical tumors treated with CTX vs CT showed downregulation of Gata4 (Fig. 7A), Lhcgr (Fig. 7B), Cyclin A1 (Ccna1) (Fig. 7C) and upregulation of extracellular matrix compounds such as Sgcd (Fig. 7D), Mmp24 (Fig. 7E); genes related to cell growth suppression, Grb10 (Fig. 7F), Rerg (Fig. 7G); G0/G1 Switch 2 (G0s2) (Fig. 7H); Tumor Suppressor Candidate 5 (Tusc5) (Fig. 7I) or GPCR-mediated Ras protein-specific guanine nucleotide-releasing factor 1 (Rasgrf2) (Fig. 7J). The gene discussed is LGR6; the disease is adrenal cortex neoplasm.