In immunoblot analysis of a panel of ALS variants at the G93A position (G93A, G93C, G93D, G93R, G93V), it was clear that the antibody exhibited the greatest binding to denatured G93A SOD1, with moderate levels of reactivity to denatured WT (G93) protein (Fig 5). The gene discussed is SOD1; the disease is amyotrophic lateral sclerosis.