Specifically, we predicted a FVIII epitope recognized by five TCRs whose TCR β CDR3 sequences were obtained by multiplex PCR [46] (Adaptive Biotechnologies, Inc., Seattle, WA) of cDNA from FVIII-specific T-cell clones that were isolated from hemophilia A patients who developed an immune response against FVIII replacement therapy [47] (Fig 4). Here, F8 is linked to hemophilia A.