RREB1 and obesity due to melanocortin 4 receptor deficiency: A number of methylation differential sites were detected in many of the genes with 26 differential CpG sites within KCNQ1 and 24 within RPTOR. The following genes showed differentiation at five or more CpG sites: KCNMA1, MACROD1, NAV1, CADM1, GALNT10, SMAD6, and RREB1 and three differential sites were identified within FTO, which is the obesity susceptibility gene with strongest effect in healthy individuals37 (Supplementary Table 2).