Previous studies have quantified the effects of specific knockdown or overexpression of either DGAT1 or DGAT2 in liver and have shown that DGAT2 activity is primarily associated with the development of hepatic steatosis (39–41), whereas overexpression of DGAT1 specifically increases TAG secretion by the liver, and increased lipid accumulation in the smooth ER (31). This evidence concerns the gene DGAT2 and fatty liver disease.