CD14+ monocytes isolated from HD were co-cultured for three to five days with AML cell-lines (OCI-AML3, HL-60, and MOLM-13/cAML) or primary AML-blasts (pAML) that were previously labeled with a vital dye for better discriminating both populations in ultra-low attachment surface plates allowing full recovery of monocytes. The gene discussed is RUNX2; the disease is acute myeloid leukemia.