MAPK8 and urolithiasis: ROS can mediate the urolithiasis-related expression of specific genes and protein synthesis and lead to renal epithelial injury, which is involved in crystal deposition [23,24], In the present study, we found that ethylene glycol could increase ROS levels, oxalate (the urinary stone-forming constituent) contents, P-JNK and KIM-1 expression, and these findings are consistent with previous results.