To test whether fibroblast-derived exosomes could induce drug resistance in differentiated CRC cells, we treated CD133−/lo CRC cells with purified exosomes instead of CM, and found that both SW620 and XhCRC CD133−/lo cells treated with exosomes generated more spheres in a dose-dependent manner (Fig. 2b). Here, PROM1 is linked to colorectal carcinoma.