The expression levels of aPKC-ι and GLI1 were significantly associated with advanced TNM stage (χ2 = 19.965, 15.458, respectively; P < 0.001), lymph node metastasis (χ2 = 13.125, 15.044, respectively; P < 0.001), and poor tumor differentiation (χ2 = 29.154, 15.273, respectively; P < 0.001) in GBC (Supplementary Table S2). The gene discussed is GLI1; the disease is neoplasm.