Collectively, our study comprehensively investigated the anti-cancer mechanism of FIP-nha using a proteomic approach and found that FIP-nha negatively regulated the PI3K/Akt signaling pathway, consequently inducing G1/S and G2/M cell cycle arrest by upregulating p21 and p27 and downregulating cyclin B1, cyclin D1, CDK2, and CDK4. The gene discussed is CDK4; the disease is cancer.