To confirm the memory identity of MSP121-specific FCRL5hi B-cells detected after resolution of the infection, we isolated MSP121-specific B cells expressing either high levels of FCRL5 or not expressing FCRL5 (i.e. FCRL5hi and FCRL5— MSP121-specific B cells) from the spleen of P. chabaudi-infected NIMP23→Rag2-/- mice (155dpi) (Figure 6—figure supplement 1), and MSP121-specific B cells from the spleen of naïve NIMP23→Rag2-/- mice, by flow cytometric sorting, and performed mRNAseq analysis on these three sorted cell populations (Figure 6E). This evidence concerns the gene FCRL5 and infection.