PKM and pancreatic neoplasm: To verify that Pkm2 was deleted in tumors from KP−/−C;Pkm2flox/flox mice, we analyzed genomic DNA from KP−/−C;Pkm2+/+ and KP−/-C;Pkm2flox/flox pancreatic tumors and confirmed that PKM2 deletion had occurred in tumors arising in KP−/−C;Pkm2flox/flox mice (Fig. 2d).