HDGFL2 and malaria: While RDTs and microscopy are considered the current diagnostic standards for malaria, the limit of detection (LoD) for each tool, 5 parasites/μL in expert reference laboratories and 20 parasites/μL more generally for blood film microscopy; 100–200 parasites/μL and 800 picograms (pg)/mL histidine-rich protein 2 (HRP2) for P. falciparum RDTs, is not sufficient for detecting low density infections [4–10].