Doing a differential expression between the two clusters of cancer fibroblasts, we found metastatic fibroblasts were found to express higher levels of soluble factors compared to primary fibroblasts including, CXCL12, S100A6, S100A10, SFRP2,SFRP4,IGF1, CXCL14, ANGPTL4 and IL6 (Table 5). This evidence concerns the gene SFRP4 and cancer.