In the multivariate model, time-varying viral load (HR: 1.84 per 1 log increase; 95% CI: 1.33–2.54; P<0.001) remained the single predictor of OIs, whereas baseline CD4 cell count <50 cells/μL as compared to >200 cells/μL (HR: 0.40; 95% CI: 0.20–0.80; P = 0.010) and time-varying increased hemoglobin level (HR: 0.81 per 1 g/dL; 95% CI: 0.68–0.96; P = 0.015) were associated with a lower risk of OI development (supporting information, S6 Table). This evidence concerns the gene CD4 and osteogenesis imperfecta.