To determine if the inhibitory effects of Arl4d on IL-2 production in T cells would have an effect on in vivo immunity, we co-transferred equal amounts of sorted CD8+CD62LhighCCD44low Arl4d-deficient (CD45.1) and wild type (CD90.1) naïve OT-1 CD8 T cells into congenic recipients and followed their expansion and function upon infection with an OVA-expressing adenovirus (AdGOL). This evidence concerns the gene IL2 and infection.