To determine whether such nuclear receptors may contribute to FSK effects towards BC formation and gene regulation in HepaRG cell cultures, we treated HepaRG cells by DDF, a structural analogue of FSK activating PXR, but not adenylate cyclase31, and by rifampicin and GW4064, reference agonists of PXR and FXR, respectively33. The gene discussed is NR1I2; the disease is breast cancer.