In good agreement, in our studies, HFD caused an elevation of FGF21 levels, associated to increased FGF21 mRNA expression in liver and WAT, while VCE-004.8 administration normalized serum FGF21 concentrations and iWAT FGF21 gene expression; the latter is compatible with an alleviation of the state of FGF21 resistance linked to obesity. This evidence concerns the gene FGF21 and obesity due to melanocortin 4 receptor deficiency.