13 representative candidates were retested at the original screen concentration of 10 μM; of these, 4 compounds (28.6%), which are β-Chloro-L-alanine hydrochloride (alanine aminotransferase inhibitor), LY-294,002(PI3K inhibitor), oxytetracycline (ribosomal protein synthesis inhibitor) and fusidic acid (protein synthesis inhibitor GTPase coupled), showed significant inhibition activity of AFP+/CD133+ HCC population without damage on hepatocytes [Fig. 2B]. Here, PROM1 is linked to hepatocellular carcinoma.