Further, the proliferation inhibition in A549 cells was induced by evodiamine, which was linked with the ability of evodiamine to increase the expression of oncoprotein metadherin, promote oxidative injury, arrest the cell cycle, and regulate the tumor-associated genes expression by controlling protein kinase B/ nuclear factor-κB (AKT/NF-κB) and sonic hedgehog/GLI family zinc finger 1 (SHH/GLI1) pathways [30,31]. This evidence concerns the gene AKT1 and neoplasm.