In addition, we classified CRC patients into four groups according to their combined expression status of POFUT1 and NOTCH1 as follow: high POFUT1/high NOTCH1, low POFUT1/low NOTCH1, high POFUT1/low NOTCH1 and low POFUT1/high NOTCH1. The threshold value which allows to classify individuals in the high and low groups is the mean expression value of POFUT1 and NOTCH1 in healthy patients. The gene discussed is NOTCH1; the disease is colorectal carcinoma.