POFUT1 and colorectal cancer: To demonstrate anti-POFUT1 antibody (ab74302) specificity, we performed an immunofluorescence detection on two human colorectal cancer cell lines (HCT 116 and SW620) stably transfected or not, with shRNAs targeting POFUT1. As shown in Figure 2B, HCT 116 shPOFUT1 cell line, whose POFUT1 expression is 30% lesser (quantification by Taqman probe qRT-PCR method), has a lower staining compared to HCT 116.