In summary, global proteomic analysis revealed that ghost pepper induced apoptosis in human renal adenocarcinoma cells was mediated through intrinsic and extrinsic apoptotic pathways, Ras, Rb/E2F, p53, TGF-beta, WNT-beta catenin, and calcium induced cell death pathways (Fig 6). This evidence concerns the gene RB1 and papillary renal cell carcinoma.