AKT1 and neoplasm: The orthotopic tumour described had higher rates of vascularisation, migration, angiogenesis and metastasis compared to the subcutaneous tumour.48 Indeed, inhibition of AKT signalling with MK-2206 in orthotopic PC3 tumours resulted in a significant reduction in the tCho/water ratio using in vivo 1H-MRS and this was due to a decrease in PC levels as shown in the MRS analysis of the tumour extracts.