To further investigate the role of the miRNAs hsa-miR-29c-5p and hsa-miR-135b-5p in 2D and 3D cultures of gastric cancer, we modulated the expression of both miRNAs in three different GC cell lines and measured the expression of the protein coded by their target genes DNMT3A, CDC42 (for hsa-miR-29c-5p) and APC (for hsa-miR-135b-5p). The gene discussed is APC; the disease is gastric cancer.