Although others have observed JPH-2 loss during HF (Minamisawa et al., 2004; Wei et al., 2010), we did not presently observe reduced JP-2 protein levels in our rat HF model (Figure 2—figure supplement 1), suggesting that JP-2 downregulation is not a prerequisite for reorganization of CRUs (Figure 2) or t-tubules (Figure 7—figure supplement 1). This evidence concerns the gene JPH2 and hydrops fetalis.